Version 2.0
D B T G R

DataBase of Tunicate Gene Regulation
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About DBTGR

Ci-crystallin

Gene: Ci-crystallin
Ghost cluster: 5710
Reference:
  • Shimeld, S.M., Purkiss, A.G., Dirks, R.P., Bateman, O.A., Slingsby, C., Lubsen, N.H. (2005). "Urochordate betagamma-crystallin and the evolutionary origin of the vertebrate eye lens." Curr Biol 15:1684-1689. (PUBMED)
This construct is available from Prof. Kusakabe.
Detected expression Expression was detected in otolith, adhesive organ.

Construct description 5'-end primer with a EcoRI restriction site: CAGAATTCTAATTCTTACTGTTCGGTTGAAACTC
3'-end primer with a BamHI restriction site: ACGGATCCAAACTTCGATTGTACAAAATGCG
Vector: pBluescript-EGFP
Insert size: 1044 bps
Insert sequence:
TAATTCTTAC TGTTCGGTTG AAACTCTTGA ATCCACTATG ACGTCATCAT CGTCGCTACA ACCGCTATAA ACAGCGAGAA ACAAAATAAA AACAATCGAC TTCATTACGG AAGAGTTGGG TCGAAAGTGG TTGTTGCATG AAATATATGT GTGTTATATC TCGGTGCTAA GTGGTACAAC AAACAAATAA AACGTCTGGT TTATGAGATG CTCGCGTGGT ACGGGCTATG TGATGTCATA ATACCTTTGT CTATTATCCT TGTTACGACA TAATTACCAT GAAACAGCAT TGTGACGTCA CAGTGCTGTT TGTGTTGGAC AAACAACACG TGGTTTCTAC GAGGGGGAAT CCCCTCTCTG TCATTATTAC GTATGATGAC GTATAATATC GATTATTATT CACCATCCAA GGTTATATAC GATATCTATT TGGTTTTATC CGCTTGGTGG CGACATCAAT CCTCAACTAC AATTGGTATT ATGACGTAGT TCGTAGTTAC GTCACAAGTC ACACAACAAA GAATTTATTA TGAGCAAATA AACGAACTAA TATGACGCAA CAATAACTAA CAATTTATCA CGTGATCGTT TTATTTATTT TGTTTGTTAT GTCATAACCA CTTGGTGCTT AAAAGGGAGC TTATGACGTA TTAATGAACT TTAATTATTA AAACAATCTT GCTTATCGTG CTGTGTTACG TCATAATACA TGCATTAGCA ATTACTTTGT TTTGTCATAA TATGAATTAT GTAATTATCG TGACGTTGTT TGTTTTAAAA ACCCATTTAT TATTACGTCA TAATACTTTA GATGGTTGTG TATGTTACGT CATAATGTTA ATTGTTGAAT AAAAATGAAG AAACAAAGCC ATGAAGAAGG CGGTTTGTTA TAAAACATTG TTGAAGGGAT GGAATTAATT ATGTTGTTAA CGTTTGAATT ATTGATGTAA CAAGGACGAC AAGTGAGCGC AAATCAGATT TCGTTTACTT TCCTTCTAAC CCTCCTAACC ACTGCTTATT TCGCATTTTG TACAATCGAA GTTT