Version 2.0
D B T G R

DataBase of Tunicate Gene Regulation
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About DBTGR

Ci-VGAT

Gene: Ci-vGAT
Ghost cluster: 6714
Reference:
  • Yoshida, R., Sakurai, D., Horie, T., Kawakami, I., Tsuda, M., Kusakabe, T. (2004). "Identification of neuron-specific promoters in Ciona intestinalis." Genesis 39:130-140. (PUBMED)
This construct is available from Prof. Kusakabe.
Detected expression Expression was detected in brain vesicle, visceral ganglion, neurons on the anterior nerve cord, dorso caudal neurons.

Construct description Name: pCi-vGAT-EGFP
5'-end primer with a SalI restriction site: TATAGtcGacTGATGGAAGATGGGACAC
3'-end primer with a SmaI restriction site: TTGCccCgggACTAACAGAAGGTACCTATTC
Vector: pBluescript-EGFP
Insert size: 2666 bps
Insert sequence:
TGGTGGAAGA TGGGACACCT TTTCATTCTA TTTTCCCGTC CCATTTGGTA GTAAACAAAG AAAACGCAAA GAATAAAATA ACCGTATCCT GACGACTACT ATAGATCGTA GTTAATTGAT TAAAACCAGT CAAGATATTC GGATATCATG ACTAAAAGTG TCACATCTTC CCCACCCTAC TATATGTTAC AAAATTTCTT TTATTTAAAC AACCAGATTG CTTCTGTCGA AGTTTCATCA AGAAGAAAGA AGCAAGTCTT TGTAGAACGT AGAGAGGAAC CACAGGTAAA ATAATAGAGC GGAGAATTTC TCTTTTTGTA AGCCATGTGG TACAGGCCGT GAGGTATGTC GTTTTCGACA CTTCACAACG AGCGTGACTC GATTCGCAAA GAATCACATT TCAAAGGTTT TTTAATAGGC ATGTTTGCAA TTATATGGTT GGGAGGATAA TGTAGAGCCC TAAATGTTAC ATTATCGAAG GATGTAACGC TGCTGTGGTA TTTGTATGAT CATTTTATTA CCGGTACGAA ATTTACATTA GCGTGCATGT GTTCCTAATT AGTTTGGGTC CAGAATTCGT AGCGTTCCGC AACGAAGACT TATTACATGA AGCTTATAAC CGGGAAAGTT GATTGATTGC ACCAGGTGCA TCTATATTGG GTTTCAGTGA AGGACTTTTA AGTCTCAATT ATTCATCGAT TTCCTGTTTA TTCCGCTCCA TACCTTCTAC TAGCCGCAAT AACCCAACGA TCAAACGACG TTGTCGGTCC GCCAAGCTTA AAATTAGTGT AGAAAGAATA CCAGCGCCGT TCGTGTTTTG CGATGTTTTA TCTTGATTTC CGAATTTATA TGTGGCGTAA GACATTGGTC AGAAAAAGGC AATCCTAAAA CATTTTTGTG TTAACGTCCT CCCCACTATA GCGTCATCTC TATTTTGTTA TATAAAAGCG GCCATTTTTT TTCAACCCAA TACTACGACT TCGGCAACCA AAGCAGAAAG TTATCCAATA TACTGGTTGC GGTCTTCATA ACGTCTTATA AGTAGTCGCC GAACTCTTAC AGGACAAATA ATTATTCTGT ATTGTTTCAG ACCGACCCAT CCATAATCAC ATCAGCCGCA AAGTACTTAG CACGAATATC TGAAGAGTGA AAATCGTATG TATTTTATCG CTAGTTTGTA TAGTTTATCA TGAATTTAAA CTACGTTTCA CTCGCCGTTG ATTTTATGAT CGCATGATGA AAATATAGTC GTCAAAAATT TTTCATGTGA AAAATATATG TTACGAAATA CGAATGAGTT ACTACAATGT TATTCCGTGA GCTTTAATAA TCCCGTGCGA TCCCCTATAA TCTATATCAT CAGGCTTGCA GGATAATACA GGTTTGGAAT GAACGAAAAT CGATCGATTG GCTACGTAAT AATCCCATGT CTTTCTATTT CACGCTTAAA TGGGCAATGT TACGCCAGGG GAACCTTTAA AACGATAGCC AATCAGTGCT TAACGCACCG CAGCTATGCA ATCGCTATTA GTGCCCCATC TTAAGCAACA ATTCCGATTG ATTCGCAATT AAACACCGAT TTGTTTTATT GGTTCTAAAT GAAAGTTACC ACTGGAGAAA AAATGTATGG CCATGTAGTC TATACTGCTT AAAAAGCGCA GCTGAATCGG CTGCAGTTTG ATTTCGTTTG TGTTTCGCAG TAGAAAAAAC GTTGTACAAG AAATTCAGTA ATTCCGGAAA AGACTTTTAG TGAATCTGCA GCGCATTCCA AATTATTGTA GGTGAATCGG CTGCAGTTTG ATTTCGCTTG ATTGTCCATG ACACGTAGTT TAAATAAGTT GCCAAGGATC GATTCCGGAA AAAAAAACAT TTGGTGATTC TGCAGCGCAC TACCAAATCA TTTATTAGAA ATTTTACATG CGCCTGTTTT GAAAATACAA TATTTTCTTG AATCGATACG CAAGGCAGGT TCTATAAATA ATTTTGCAAA ATACCCAAGC GATGAATGAA GTAATTACTG CCTGGAATGA TTTACAGAAT CCTGTTACAG TCAGAGCATT TCTTTAGAAA AGCAGTCCGT TGATGTTGTC AGTCGTAATT ACGGAGCTTT TTGGCCCAAG ACATTTTCAT CAGCGGTATT CGGAGAACAT GGTTCGCCTC TCTAGCTGTC AACGCACGAT TCCCTGGAGA TAAGTGCGTG GCTCTACGCC TCTTGTCAAT GTCGCTATAA GTGGGCTAAA TGAGCGCTTT ATTGGGGTAT CTGCGGTCGC AGAGATTCAC CTGCATTTCG ACGCATTCGC GGACCTCCAG CTCGCAGCGT TGATACCGAT CGTCCCTGGT GCGGATTAGC GTAGGCAAGA ATATGCCTTG CTTATTGGTC GCTCAGGCGT GTGATCCTGC AATCATACGA TACGAAGACA TTGATCGACC AATCTCAGGC TATATTAAAC AATATTAAAC ATACTGCATT TATAATAGTT CAGTCCAGGC TGTATATTGT GCTAAATAGA ACCAAACTTA AACAGGTTTT TATTGAAAAG GTAGCCCAGT TCGTGCGATA AAGGCTTAGA AAAGACATAG TCTAAGAAAT ATTTACTTCA ACCAGACCTG CGTGGTGGAA TAAAAGGTTA TTGGAATAAA AATAAGAATA GGTACCTTCT GTTAGT