Version 2.0

DataBase of Tunicate Gene Regulation
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Gene product: NK-2 class
Organism: C. intestinalis
Summary of experimentsThe minimal endodem-specific enhancer region lies between -355 and -36 (from TSS). (Fanelli 2003)

The following regions have been identified (Fanelli 2003):
From -355 to - 283: expression enhancement
From -283 to - 235: repression of the expression in mesenchyme
From -235 to - 130: activation of the expression in the endoderm
From -152 to - 36: activation of the expression in the mesenchym

2 DNA fragments are bound specifically by protein extract from larvae (Fanelli 2003):
S2: from -209 to -199 (GCATTTGATTT)
S5: from -266 to -262 (GCGCT)
Activity reported

An unknown factor regulates Ci-titf1 in endoderm in C. intestinalis (6, 25, 26)
  1. Kusakabe T. (2005). "Decoding cis-regulatory systems in Ascidians." Zoolog Sci 22:129-146. (PUBMED)
  2. Ristoratore, F., Spagnuolo, A., Aniello, F., Branno, M., Fabbrini, F., Di Lauro, R. (1999). "Expression and functional analysis of Cititf1, an ascidian NK-2 class gene, suggest its role in endoderm development." Development 126:5149-5159. (PUBMED)
  3. Fanelli, A., Lania, G., Spagnuolo, A., Di Lauro, R. (2003). "Interplay of negative and positive signals controls endoderm-specific expression of the ascidian Cititf1 gene promoter." Dev Biol 236:12-23. (PUBMED)