D B T G R
DataBase of Tunicate Gene Regulation
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About DBTGR
beta-tubulin
Gene: Ci-tubulin-beta-2
Ghost cluster: 86
Reference:
Kusakabe T., Yoshida, R., Ikeda, Y., Tsuda, M. (2004). "Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona." Dev Biol 276 :563-580. (PUBMED )
This construct is available from Prof. Kusakabe.
Detected expression
Expression was detected in neurons .
Construct description
Name: pCi-tubulin-[beta]2-EGFP
5'-end primer with a SalI restriction site: ACGTGTCGAC GATCAAGCACTGAGGGTGCT
3'-end primer with a BamHI restriction site: CGATGGATCC TGGCCAGCTTGCAGATGAAC
Vector: pBluescript-EGFP
Insert size: 4336 bps
Insert sequence:GATCAAGCAC TGAGGGTGCT TATTGTTACG AAATTAAACA GAGTTTAAAT TTTACAATTG TATGTAGAAC TTTTGAAAGT ATAACATGCT GTATTTTAAA TGTTATTTTT ATACTTTAAT TTGCAAAACT AGCGAACTCC GCATTATGCA AATCATTTCA AAACTTTTGT TAAAACCGGT AAAACAACTG ATAATTTTGT AATAACTCAG ACTTACAGTG CTGCTATTGG CTGTTTGTGA AAGTTCAGTT TGTTTACAAT TTTTGTAATA ATTTGATATC TGTTGCCTTA CCATTCATAA AAATGAATTT AATACCTTGG CTCACTGTTT TTGTAGTACT AGTGTTTTTA TGTTGTTTAA TAAACTGATA ATGAATTTAA AGTTGGAAGT TACTGTTCTT GTGAAGGAAT TGTCCATAGC TTAGTGGTTA GGCACTCGCA TTGGACCAAA GGTTGCTAGT TTGAGACTCG ACACTGATAC CGAATATGTG TGCAAAACAA CATTAATCCA ACTACTAGAT CACTAATAGG TTGTCCAAAT TTTCAGCCAT ACAAAACAAA ATAAATTAAA AATTATCACA TGTTATTACT TCACCAGCAA AGTCAAAAAT GTGGTTACTT GTTAGCCGAA ACAAGGTGTC TGAAACAAGA AGCCTGTACA GACTTCGTTG CGTGGATGCA ATAAATCTTA TTCGTTGATA GGTGTGCCAC AAAGCTGTTA TGGCGTATTT CGATTTTTAT CCGTTAGGTT GCGTTGCAGA GCTGGCGAAG CCAAAACGAC TGTTTTTAAA ATTAACTGCT AGTCTGCTTA CGGTTGGACG GTAAGAATGT TATTGATAAA CTAAGAATAG AATCTTTAAC GATCAAAATT TTAAGCACCT TAAAGCTTTA AAAGAAGCTC TTAAAAGCAA AATAAAGTGC TAAATAATTA ACTGTTTGAT TTAGGGTTTA ATTTTAAATT TAAAAGAAAC AGAACTATTT TGATAGTAAT AAAACTTTTA ATATTTTCGC AGTTTTGATT GAAAATTGTT CATGCGACGA CTGTTCTGCA CACGCTCAGG ACCAAAATGT CGAATCACGT CTGTGTGACG TAACACTGAG ACACAGACAC CAAGACTCGG TCTTGGGCGC GGTTTTCTAT CGATCGACCT GTACTTTGTA ATGGGAAGTA CCGGCAAAAC CGGGATGCAT TCTCAAATCG ATTGACCATG AATAATTTAT GCTAGGCCAA ATGCAGCGAG CGCGTGACTC AGGTATGGAT GAGAATTATG CATTAGGGAA TATAAGACGA CGCCGTAAAG TTGTACAAGT ACCAAGCGAC TGTATACAAA CAGCTACATG TGTCTAACAC CAAAATTATG ACATCATCAC ATTTATTAGG CAAGTGATTT TTTAATACTA TAAAAGGGTT GGATACGATG GAATATGTTT TTATTTTTCT TTCTTGTCCC ATCTGGTAGC AAACAAAGAA CATTCAAAGA ATTATAGAAC CGTATCCTCA CGACTTCCAG CATAGAACGT TGTTATTTGT TTAAAACACG ATCAGGATAT TTGGATATTA TATGCTAAAG GTGTCGCGGC CTCCCCCACT CTACTGTAAC AATTATAGGC CGCGACTTTA AAGGAGAATT GTTAACTGTG CAAAAAAGGT TCAGGAAATA TGGGATTTAA GTGCAAACAG TGTTATCTCA CCCCACACTA CTATATTAAC TGTGCATTAT TCCAATAGCA ACGTACTTCC AATACCATAC TACTTTTTCT AGTTTTATTA AAGCGTGATT GCGTTTTATT GTACTTACTA TTGCCAGCAG TTCAGTTTTC CCGTCACTTT TTATCGGTTT TACAAATCAC TGACTGACTG CACCAATAGC GTTTAAATCT CAATAATTTT GGACAATGGT TTTAATTAGC GATGCAGCTA TGTAGTTCAT GGTAGAAAAC GAACATCAGT AACCGTAAAT GAGAGTTTTC CAACTAATTA ATCCGTTAAT CTAGAATTAA ATTTAATTGC GTAATTCCAT TGTTAAAAAG AACTATAGCA GCTGTTGTGC GATTAAGACA ATACATTGTC GTTCGGTTGG TAGTCTCACC CACGAAGACA AAATTCGAAG GAATGAAGGT ACTTCTTTTC ATCACCAAGC AACGGAATGT TATTCCTGAC TTGATCGGGC ACGTCGCTAG CGTCGCGTCT TGTACAAATA TCAATTTCCA AGCCGAGATT GGAATTCGAT AACAAAGCGC TTCTATCGCT TTCACGGAAT TGTATTAAGT TACCATAAGT TCTGACATTT TGGTTCAGGC TTTATAGTAA AAATGATTAC CTTTTCACGC AGTGTATGCT GCAACAAAGG TCAGGAAACA GCAGATGCGT TTTATCTTCC TCAATCCACT TTATTTTCAC ATTTGTTTAA GGTTTCATGT GTACGTTGTA TTTGTTCAGA TAAACAACAG CAAAAATGAA AGCATCCATA CACTAAGTCA AGTAATACGT ATAATCAAAC TATCGAAGTT TTACGGTTTT GACGTACAAA ATTGACCTTA AATCAAAACA AAGCGCGGGG TTGGAGTTTT ATATTGTGTT GTATAAGGAA AATAACAGCC GAATTCTCGG TGTTTAGATT AACCTACATT ATTTGTGATG ATACAAAGGA AGAGTTCTCG TTGAAGCTCA AACCCCACCG TTTCAGCCTC TAAATTATTG ATAAGTTCGC GCTACGTGAT TGGATGCCGT CCAATATATC GAACCCTCAA ATAAATTACT CACAGTCTCC CTTATGACGT CATACAGACA CCCTGCACCT GTCCTCCCAA CGGGAATTCA TTATTCTGTG ATGCTGTCTT GCCGCAACGA ACACCAACTG TTGCATAACA ATGCACTGGA TTCTAAAATA GGGCTATTCT TTTTTAACAG CGTGCAGTTT ATACACTAAC ATATGGCCCG CTCACTGCAA GAAAACAAAT AAATTTGTCT AGCCTTGGTT AAATTAAGAA CATTTGGAGT TTTTACCGTA TGGAAATCGA CAAACTGCAT TATTATTAAT GGCACTTTCG TAACAATTTA ATAGCACGCT AAAATTATTG ATTGTTAAGT TATAAAAACA TCAATACCGG CACACTCGGC GTATCCACGC AGGAGTTCAT TTGTCGATAA ATTGAAATCA AGCACACTTG ATAGGCTAGA ATGGTGAAGA TTAAAATGCT AGTTACTCCC AAGTGTACTG TTTCGTCTGG GATTGTTGTA GCTTTTTATA CTTAGTGGGC TCACTAAAGA GATTGTTTGT ATTTAGAAGG ACAATTAATG AACATTGAAA CCTCTCAAGA AAATGTGCTT GTTAATCAAT TGTACAATTT GAATATGGCC ACAAGCTAGC CTTAGATAGT AAAGTTCCCA CATATAAGTA TTTAAGTTTA AGAACAAATC TATAAAAAAC ACACCAACAA TCAATATACT GCAACACAGT AACTTGAGCG TGGGCATGGG GGGTTCATAA TAGCTACACA AAGTTAAATA ACTCACGAAA ATTAAAAAGC CAAAAGCATA ACACAAATAC AGTCAAATAA ATCAAAGTCC AACATAAGCT AACGTTAAAC GTAATGAATA ATCCAATTTG TCATTATTTG CATTGCGGTA CACAGGATTT CATTGCAACA CACGGGGATG ACGACCGACT ATGATATTCA GTGTTATGTG TTACTGTACG GATTCGAAAT AAATATTTTT TGGTGTCTTT TTCGTTGACA CATTTGTTTG CGATCTTACG CCCTACAGAT ATTTAGGTTA CACTGATTAC GGCATGCTTC GGGAATTACA ACATAACATT GTCTAATCGT GCGGAAAACT CGCGCGCACG GGCAACAGAC TCGCGCTTTG ACGCGATCAG GAATCAAATA ATAGCGTTGT TTGAAATGTA AGGCGTCCCT ATAGTAACTA TTGTGGGACA TATACTGGTC ACCTTACCTG GTGTATTCTA CAATTGAAGG GTTTGACGGC GAGTTGAGGC TCGCTTGCTA TAATGAGTCA ACAGACACCA TTGTCTTGTT GCTAGGCAGC CGCGGCTGTT GATTTTCGAA GCCCGTGGAA ACGTACCACT CCGCCGAGAG AAACGAGACC CTCTCGTTCT TTGTTTAATT GATTATTCGC CGCGTTCGCG CGACAAAAAA GTTTCCTACA TTATGACGTC ATGAATATCG AGCGCCGAGG CGCGCTTAAA TATCGCGACG TGTGGTGCTG TCGTCATTCT GCGATTGAGT CGAGTACAGA GAATATTTTT ATATATTTTT AACAATCTTT AGTTTCTATT CATCATGCGA GAAATTGTTC ATCTGCAAGC TGGCCA