Version 2.0
D B T G R

DataBase of Tunicate Gene Regulation
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About DBTGR

beta-tubulin

Gene: Ci-tubulin-beta-1
Ghost cluster: 774
Reference:
  • Kusakabe, T. (Unpublished).
This construct is available from Prof. Kusakabe.
Detected expression Expression was detected in papilla, brain vesicle, caudal epidermal neuron, mesenchyme, nerve cord.

Construct description 5'-end primer with a SalI restriction site: TGACGTCGACTCCAGTGGAGCGTGAAACTT
3'-end primer with a BamHI restriction site: ACGGATCCGACGTAACGAACGTCTAACC
Vector: pBluescript-EGFP
Insert size: 5712 bps
Insert sequence:
TCCAGTGGAG CGTGAAACTT CCTTTTTCCG ACCAAGGTAG TCAAAACAAA ATGGAAGTTT TAACTGAAGA TGATGTTTCG TATTATACTC TACATTCCAA ATTACACTAT GCACTACTGA AAGCACTTTT AGCTTCCAAA CCAGCACTGA AGGTATAGTT TTACTTTTAA AATACGTCAA TGTTTATTAA CCTACTTATA TAAAATAGGG TGGGGTAATA TGGGACATTT CATTCTTTTT TCTCTTTCCG TTTTGTAATA AATAAGAATA TTTACGGAAC TATAAAACCA TAGCCCCGCG ACTCTTGAAA GAGAGTTGCA ATTGTTCTAA ACACAATCAG CAAGTGTGAA ATTTAGGAGC TGCCAATATT CCATCTTATT CTACAGTATT ATAACAGGGT TTTATTTTTT AACTTTGTTT ACAGCAGTGT CATAGTAATG TTATAACTTG CAGGCCATTG CAAAAGGATC AGTGTTGGGG AATCCCGACC TCCAGTTGTG GGGAAAACCC CAGGTTCATT CCCTGATTGG TGACCTCATG ACCTTGTGTC CAGATTCCGA TAAACCAAAG GCAGGAAGTC CAACCGAGCT GTCAATCAAC CGATTCTCAC AAATTTGTCA AGTAGGCATG ACCATGAGAG TTCTTAACTG CACTAAAGGT GAAATTTGCT ATTGTAAAAC TTGTAGCATC CTTCGTCTTT TAATAGATAC TTGATTTCCA ATTTATATAT TCTGCTGTAT ATTGTTTTCC AGATCAATTT CAAAGCCTTT CAGATCCTCT GCCTAAGACA AGGTAAATTT GAACTTTACT TGCAGCATTT TCCATGCAAT AATACTGTTG TATAGCAGTA AACAGAAATT GTCATTACTT AGGCTTCTTG AACTGGTTGC GTACGGTTCA GCTCGCAGAT CATCTGTGGA TGAAGTTCCA ATGGACATCG CACAAAGTTA TTAATGTCAA TGTACTCCAT ATGCATATCT TCATGTTCAA GGGTACTAGG GTAATGAGCC AAATATGGTG TATTCCAAGG TCGTTAACAA GGACTTCACC CATGACAAGG AATTTAGCCA TATGGAATTA AAGGATTATA CCAACTTGCG CAGATCTAAT TGCTACACTA CTATGTGGTT GATTGAAAAT GTAGTTCCAT ATGGATTGGC ATAAATACTT ATACTTAGTC CAATAAGTGT AATGTGTGTG CTGTTCCCTG GTCCGCAGTT TCGAAATGGT GAAATAAACA TATTATTCTT TATTCTGTTC ATAATTGTTA AGAACAAAGT GGTCTGCTCT GTTAGAACAA CAGTGTATAT TCCAATCGGT GTTGTTAGCC ACTTGTGGCG CTGCAGAACA GGTTAAGTAC GTCACAATAT AGGTGCAAGT CAATGCGTGT AATGTGACGA CGGTAGAAAC TGAGTTGTAT ACACTCTCAT GTTGAAGAGA AATCCATGAC GTGTTACTTA CCAATGGGCG CCAAGCCTAT ACTACATAAT ATACTGGTGT ATGGTGGAGT TGGGCATGAT GTCATTTCCT TTCAAGTGAC GATACTTCCT GTCTGCCATG GACCGCCCTA AACCGTTAAC GCAACCAATA GCACAGCGGT TGCTGATGCA CGACTATATC CTGCATTGTC TTAGATATTC TGTGTAGATG CTATGTGAAT AAGACAATTA TGTTACACCA TTGTTAGGCG AGCACGTCTA AAGGGCTAAT GTTCTGAGTC TATAAATCAT TAGGGGTATA GTTATATTTA CCTAACAATA AAAAAGAGCC AGGAAAGTCA TAATTTATAT CAAAACTTGT GAATATTGCC ATTGCTAAAA AATAATCTGT ATTACAATAG ATTAACCGTT TACCAAATGC GATCAATAAC AAATTAAATT TACATTCTTC AGCGTAGAGC AACGTGACTA TGTTGGCGTG CGGTTTACTC GGGTCTCTAT ATAGCATGTA GGCGGATCAT TTTGTGTGGG TATTTTAATC TATTCCGCAT TGAAGGATGC AGTTGCGTGA TTTATTCTTA ATTAAGCGTT GGGACTGTAT TTTAAGGTCA CGCAAATTTC TTGTTACATT CGAACTTACA ATTTTTGTTT GAAGATTTTG TAGTTTATTT AGTGTAGGTT ATTTTACAAC GAGATTTTGT TGTCGAAGTT ATTATACTTA AAGTACCGTG GGAAAAGATG GGACACCTTC AGCTCGAAAT AGTCAAATAT CCTGATCGCA TTTTAAACAA TCAACAACGG TCTATAGAAG TCGTAAAGTT ACCGTTTTGA AATTTTTTAA ATGTTCTTTG TTTACTACCA AGTGGGACGG GAAAATGGAA TGAAAGGTGT CCCATCTTAC CCCACCCTAC TGTACTTAAC GATAGCTGTT GCAAAATTAT GTTTAATTTA ATTGAAAGAG ATACTTATTT TATAGCCTCA GTGCAGGAAA TGCAAATCTT TCGGATTTAT CAGAGCTACA GAATTAGTAG CTTATTTAAG CCATGGCTTA CTAAACATAT AGTAGGGTGG GGGAAGGTGG GACACCTTTA GCACGTAAAA ACCAAATATT TAGATCGTGT TTTAAACATT TAACAACGGT CTATGAGAGT CGGAGGATAC AGTTATATGG TTATTTGAAT TTTCTTAGTT TACTACCAAA TGTGACGAGA AAGTAGAATG AAAAGGTGTC CAAACTTCTC CCACCCTACT ATATCTTTAG TGGGCCATGA TTAAGCTTAT GTGAATGTTA TGTATTTCTA CGTTAAAGTG CATAAGGTCA CTGTGTACGC ATTTCACTCA CGTCGCTCGT GTTTTTACAA CAGCCTGTAC AAAAACAGTT ATTGTTGGAG ACCATGTGAG GCCACTGTAT CGGGCTGAAT GCTTTTGGAG AATAAGAAAA ACAGCGCTGA TATGGTGTAT GAAGCATTGC ATACACAATG CTAAGATAAA CATTTTAAAT ATAAGGCGTG TGCATCTTCC CGCTCTGTTT ACATTAATGT ATCACGGTTG GTAAACGTGA CAAGTTGAAC CAATGTTGCA AACTAACAAA TGTCCCCACT TTAACCGCAC ATAAAATTTA ATATTGTGAG TGAAATTTCA CAAACGAAAC AGTAGCTAAA TTTCCTGGAA AAACATCCCG TTTATTTTAG CCTTTACATT CAAATCGCTT TAGATTATCA TAACAGCCGC ACCAGACACA TTAATACGGT ATAGTTAATC TCGCATGCCA CTTGTCAGCG TGAGCAACGG TTTTAGTAAC CGGATGTACC AATTCATTAT ACGTATCAGC GTTTTGTTTG AGATTTAGCA AAAATACAAA CAATATTCGA ACTTTGAACT GCCAATAAAT TGGACAACTG CATAGCATTA AACACATTAT ATTGATATTC TACTGCGAAG TTCGGATCTT TACTATTGTG TTAAAATAAA TGGGTAGTGT ATATGTGCTG GCTTGCAGTA TAAGAGAATA CAATACTGGC GGTTAATGAT TTTTTTTTGT TTAACTTAAC ATTAACGCAT CTCTGGTTCG TTAGCAGACA TTAAATGGAT TTTTACATCA GCGGTGTGTG GTAAAGACGC ACTGATTTTG TTCCCAAATG GGCACGTCGT TTTTAAACCA GTTTTCATTA AATTTTTATT AAAAGTAGCA AGGTTGCAAC CACGGTGTTC ATAGATGAAT TATAATGTGT AGAATCTTCA CATAGAGTCT TAATGGTCAT AATGAGGGAG CAATGATACG GAGAGGGGAA TTAAATTAGC ACTGGGAAGC TATTACAGGC AGGTTTGCCA TATTGAGGTT TTAATAATCG ATGCGCTCAT GAAATATGCA GGAGAGGGAA GGATTCAGAT CGAGCGGCGT TTTGATTTAT GCGCGGCATC TCTGTGAGAC TGAGATTGAA AAATGGAGCA AAATACAGCT CGCCAGCTTG GGCAGCAATA TTGCCATCAT TAGGTGAAAT GGCCGGTAGC ACAGAATGCA ATTACTCGCT CGCAATATAC TGGCATATGG CACGCGCTCC ATAAATTACA TCGCTGTCTC CAAATAATGA AAAGGGATCT CGGTTCCTGC TGTGCCCGGC ATGTCATGTA ACGATTTTCC ATGCGCAGCA TATTCGGCCG CACAATTTTC GCTTTGCACT GCAGATTCGA TGATGGGAGA ATTCAATTGT TAAAACACGA CGTTAGTTGC GTGAATCGTG CGCTGTATAC CTAATGTGGC GATAAATGAA TTCACGCCTT CCGCCTAACT GACGTCAATG GGAAAATAGG TGCTTCTTTA CCAGTACCCG AAAGCGGCGC CGAAACCAAC ATTTTGGTTG CGATTTTACC GCTGTAAACA ATAACGACAG AAGTAAACGT AACTGCATTG TTTCTATTAG ATGTCACAAT ATATTTTATC CCTACGAAAC TTTTTGTAGC GTTTTCTTTT ATTTGAAAAA CAGAATTATT GGATTTTTGG AGAAATTGTT CATTTTTTTC ATCAAAATAA AACTGATTTT AAATCACGAT TTTCAACAAG GATTGTAACT CGGAAGCGTG ATTGCGTAAG CCCAGTTGCA GGAGGGTGGC ATTTCCGCGT ACCAAAGTGT TGTAGTGGTT CATTCTGCGC ATGTCATGTA CGTCTTGTAC GCGGAAGTCG AGTCCAGTAA AGCCCGATAT CTTATGAAGG CATGTTTACT AGATGCGATA TCAGTAAAAC TTGTGGTAGG TAAACACATC TTTTTCCTTG GAAAGTAATT CTATGTTGTT GCTCCTAGTT TAGACTTGTG GGTCAAAAGC CAAGATGAGA GTTAGGAGAG TTGTTAGGAT CAATTTCATA TTTAGAATAG AACTGGAATT GTGAGATTTG TATTTTGTTT CAATAAATGT AAATTGGACG TTACAGAAAT CCATATAGTT TCAGTATAAT AGGGTGTATA CGCCCGATCG ATATAGCATT TAATGCTTCC AATGCGCTCA AAATACGTTT TGTGTCAGAC CGAACAGTGA GTTTGCATCG TGCCAGCAGT TTATCAGCCG CAAGCAGAAA AGCCAGGGCG CCGTTAAAAG TGCAACATAA TCACCATCTC ACTTGATTTC CCGGCTTTAT TTAGGTTAAC TACTGATAAA GCCCCTGGAC ACACGTTTAT TTTGCACCGT GATTCCAGTG ATGGCCTAGT TCGAGGCAAA AGTTGAATTA AAAAGCGATA ACATTTGAAC GGAATAAAAA TTAAATCACA GATAACCGAA TTCAGTGTCG AAATAACGGC AGATGCGAAG TTTTAGGGTA TTCGTTTATA ATACGCTTTC CCGTGTTATT TACCAGTTCA TTGAACAATG ATGTATTTTC AATTGCCCGT CGGTCTTACA GCAAATCAAT AGTATTGGTT TGTGGTGTGT TTTATGCCCT CAGATTCTCC CTTTACTATT TGATTGATTT CGAGAACGAG AAGCAATAAT TAATTTAAAC TTGTTGTTAC CACGCTGCTG ATTAAAATCT GAGAAACTGT TGAGTTGCGA TAGATAAATG GATTTGAAGC GATTTTTATT TATAGTTACA AGTTTGAGCC TCGTCGCAAA GCGTAATCTT GATAAGAACA ACGCGATTTG CGGCTTATTG TTCTTCTTTA TAACTTCACT GGGAACCATT GTTTGCAAAA TTGAGCTCGG ACCGATTGCT TGCAGTTTCG AGCCGCAAAT AATCGTCCCC CCATTGTTTT ACTTTTGACA GAGGTTAGAC GTTCGTTACG TC