Version 2.0
D B T G R

DataBase of Tunicate Gene Regulation
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About DBTGR

Preprogonadotropin-releasing hormone-2

Gene: Ci-proGnRH2
Ghost cluster: 7965
Reference:
  • Kusakabe, T. (Unpublished).
This construct is available from Prof. Kusakabe.
Detected expression Expression was detected in visceral ganglion.

Construct description 5'-end primer with a SalI restriction site: TGACGTCGACAGGAGCAGACGTCATAAGTA
3'-end primer with a BamHI restriction site: ACGGATCCTGTTACGTTATCTCTCTAGAAG
Vector: pBluescript-EGFP
Insert size: 4320 bps
Insert sequence:
AGGAGCAGAC GTCATAAGTA GATTATGACG TAACAAACAA ATTGTGATAA GGCAAACGCC TTCAAGTCTA AGATTGCACA ACAACAGGAT ATTTTATCAT TTTTAATTCG GTTTCTGAGC CATGAACGCC TGGTGCATGT GTTACATTCC TTGTCTAACA GAGACACTGT GTTTTCTTTA AGCGCGTGTT ACCTACTACA ACACATTACA AGATTTCTTT ACTTTAATGC GGCAAAAGCC AGCGAGCATC GGTTTTATGA CGTCATATAT CTTAACGTCT TCAGCATTCA TATAAATATT TAGTGACGTC ATTTGTGTGA CGTATGTCTC GTGTTGGTTC TTTTAGTTGA TTGTTTGAGT TGATTATCTT CAGTTGCTAA TGTACAAAGC AACGAATAAA GTTTCTTCAA TTTTGACGAA TGTATGAATG AAACTTATTT ATCCACGCGT GGTGGGGAAA CGGTAGTTAT AACACTTCGT TATAAGAAAC GGTCGTTATG TCTGTAACTT ATTTATCCAC GCGTTGCAGG GAACAGACAG TCATTATAAG TTTATAACAC GGGTGTTCCG TTTCATACAT TTTTCATACC ACATATATAA CTTTGCGGGT AACTGTTTTC AAGTTTTGCT GTATCCAAAG GGTCTAAAAC GTTTGTGTAT TCGTCTTACA ATGAACTCCA TTTAAAAGCA CCGACAATTG AACCGTAGGC ACGCAAGAGC ACTTGGTACC ACTTCAAAAT CCAGCTGGTA CGATATGTAT TTAATAACAT TGGGATTTCA AATCTAAATC ACGGTCTTTA TTCCGTTGGG AAGAATTCTT TATCATGAAT GGTCACGTGC TTCTCACCTT GTTACGAAAT AAACGGTCGA TTGAAGATTC AAACGTAACA ATAGGCATTG TGATCAAGCA GTGACTTGTT TATTTTTAAA ACCCTTTTGC TTTGGCGCCT TTGAAAACGT GTCTCACAAT GAAAATCCTA TGGAATTACA TTTTCCCATC GTTATATGCT GTTTATATTC ACCGGTACTA TCTGGATTAG CTGAGTTTTT CCAAGCTGCT AACCTGGTTG CATCTAGCAA TGGAATTGCG GCCCTAACCT TTTACAGTTA TTAGTAATTT TGTAGAGATA AACCAAACAT TGTTTAACAA TTTGTGAAAA CATTTTTAGC GGTTTTATTG TTTTTCGTCG TGTCAAAACT TGTTCATATT GTGCACGTAA TTCCCCCGGA GACATACTTT CTTGTAACAG ATGCAGCGTT AACGTGGCAC GGTAATATTG CGGTTTTATC GCTAACACCT GTGTCTAGTG GTTATGGTTC ATGATCCAAC ACACGGTAAG AATGCTGTCC GTCTTCATAG CTGTTTAAAG GCTACAGTCG TGGCTATGTG ACTTCTAAGA TTTGACTGTC ATCTAAAAAT GTGTGTTTTC AGGGAAAATA TTTTGAAATA TCGAGAATTG TATTACCTTT TGTGATGGTT CCATTTACTA GGATTGTATT TGCAGTGTTT TATTTTACTG CAGCATGGCT CTCGTAACCG TGCATCAAAC TATAACCTAC CTTTTTTTCA TAATTCGTAC CCTTACTTAT AGAATAGCCA AATATTTAGT CCAACGCCGT GGCTTAGTGG TTCGGTAGTT GCATCTTGTA AACGTTATGC TGCTATACAA AGGTTAGGGT GGGAGAGGAC GGGACACCTT TAGAACATCA TATATCCAAA TAAACTGATC GAGTTACAAA CAATAATAAC GGTCTGTAGA AGTCGTGAGG ATACGGTTTT ACAATTCTTT AAATGTTTAC ACAGTTTACT ACCAAATAGG ACGAGAAAAT AAAGTATAAA GTGCCCCCAT CCTACTGTAT TTCCTAATAC CTTGGTGTAC GATTGACAAG TTGGAAGGCT CTTAAAGGAT AAACGCTGGT TTAAAAGTCA TCTCGTCATG ACGAGTGCAA TAATATTGGC AAAAAATCCG CTTTTGAATA TTTTATGCAA CAATTAAAGT GATACCAGCA TGATGTCCTA CCGGTTGCTG GTTCTAGGAT TACATGCGGT ACATCCATCA TGAGCAAAAT CCAACCCTTT GTCCTGTATT AATATTTAAT GAACTTTTGA CAAGAGGTGC TTGTTTTCAT TGTTAACGAG GTAGAATAGA TATTTACTGA AAACATATCT CTTGTTTTAT CACGGAAGCT CATGTTCTGG AGGATTCTCT TTACCAAATT ACGTATACTC GATAATCAAG GCAGGGGCGG CTGTTGTTAT ATGATTAAAA AGTAAAACTC GTTAGGGCAT CAAATCGGAG AATTGAGCTG CCGAATTGTC ACCACATATA ATAAAACAAC TTTCTCCAAA CCTATATAAC ATACAAGCAA GCACGAAGCG TATGAAACCC AATACCTGTG TTAGAACAGT CGTTTATCCA CCACGAGGGA ATAAATAAAT TAAAATCAAT CAAGAATACC GGTGCATACG TATTGGGCGA TGTTTATAGA GTAGGGTAGG GAAAGATGGG ACACCTTTTA CCTCTATTTT CTCGTCCCAT TTGATAGTAA ACAAATAACA TTCAAAGAAT TATAAAACCG TATCCTCACT ACTCCCATAG ACTGGTGTTA AGTGTTTTAA ACACGCTCAG GATATTTGGA TATTATGTGC TAAAGGTGTC CCATCTCCCC CCACCCTACT ATATGTATTT TCCATGAAAT TATGCGCATC AGAAACAACC TTTACAAACT GCCACCTTGG TTTAAAAAAA CTTTAAATGA TTTGAGTAAA ATTATCTCCT CCCCCCTTTA AGTATTCGAC CCTTTTCCAT AGTTATTTCT CCAAGATAAG TTCGTAAGCT ACCAAGAAAT TAATTTCGCT GCAGGATAAT GACATCACAA ATTGTTTTAT CGATTCTCCA TATTGTTACG TCACGATGCG CGAATAATGC TCTTGTGGTG ACGTAGTTAC CAATAACATC GACTTTTGAA ATTCCTTTTG GGACAACAAA TGTAGAAGGT CACAATATCA TTTACGTCAT TGATGTGGTA AAACAGTGGG TCATCTCATC CGAGCAACGG GAGTCAGTTC AGATCGTTTG CTTGCGGCAT GGGGTAGTTT TGTTCGGTTT GTTTGTGCAT TTTCTGCCCG TCTGCGTCTC ACTGGCACCG CAATAAAATT TAAAATGCAT GAAAGGCGAG CGTATAATTG GAGTCCCGTG TAAAACTATA TAAGCACAAA CTGCAACCCA GTTAGAATAT CTGCATACAG AGAGTGTTGC AAATAGGCGA CTTTTCGTAA ATTCGGGGTT GCATATTTTG TTCGTGAGTA ATATAATTAG ACCAGTTTCT CGGTTAGGCA GAGTTATCGG TATAACGGTC TTCTGAATAA AATCCGCCTT TTTGTTTTAA AATAAGTTTG CGTAGTGATT TTGCTATAAA CTTGTCTTGT TGTTTGTGGT GGTTTGATTC CTGTATAGCG GTCTGAGGAA TATTGCTAAC TATGTGTCGT AACATGTCGT TACATACTGT GGGGTGAGAT GGGATACCTT TATCACCTGA ATCCGGAATT ACCTAATCGT GTTTTAAACA ATAAATAACG CTCTTTCAAA GTCGTTAGGA ACGGGTTATA CAGTTCGATA AATATTTTTT GTTTAACTTC CAAATTGTAC GAGAAAAAAG AATGAAAACA CGAAGCATCT TACTCCACCC CACTATATAG ATTATCGGAC CCCGAAAAAG ATGCTTAACG CAAACTAAGC GGGACTAAGA TACTATACCC TTATCCATTT CAATATTAAA ATATTAGGTT TTCTAAGCGC TTTTATTTAT TTCGTTAAGA TTTCATTCTT TTTAATATCG GGTTTAGTTG TGACAGATGT TGGTTTATTA AAGATAAAGA TTTGTTAAAG CTCGATACGT TGCCATTGCA TTAGTTTATT GCGATTGCTT TTAAGAATAA AAGTCGAATT AAAAGTGAAT ATGAGTTAGT TGGATGTGCT GGGCATGTGG TATAAGCAGT GTGTTTGAAT TTAAAATAAA GATTAGGAGA TGTTCTGGTA AATTTTGCGC GTAACAATGC ATTACTGTGG GGTAATGCGG GGACAAATCT GGCCCACGTC CCAGGTCCCA TTGTATAACT CGTCGAATAA AAGAAAAAAC TATAGCCAAC AAAGTTCGCA GAATAGCTTA TACATAAATT AAAGTATGGG TGGCAGCAAC AGCATATAAT AAACCATCGT TAGTCATTCC AGTTTGTAGA AATCCAACTA CTGGTCAATT TGTATTAACA AGAGTTTCAA TTTATTTTTC GAATTTCTCT TCTAGAGAGA TAACGTAACA