D B T G R
DataBase of Tunicate Gene Regulation
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About DBTGR
Ci-G-alpha-i1-int
Gene: Ci-G-alpha-i1
Ghost cluster: 1990
Reference:
Yoshida, R., Sakurai, D., Horie, T., Kawakami, I., Tsuda, M., Kusakabe, T. (2004). "Identification of neuron-specific promoters in Ciona intestinalis ." Genesis 39 :130-140. (PUBMED )
This construct is available from Prof. Kusakabe.
Detected expression
Expression was detected in papillal ,
brain vesicle ,
visceral ganglion ,
caudal epidermal neurons .
Construct description
Name: pCi-G[alpha]i1-EGFP(-int)
5'-end primer with a SalI restriction site: gaCtGtcgAc CTGACCAGTTTTAGCCAA
3'-end primer with a SmaI restriction site: gactcCcggg CTGAAACCAAAGGGATGA
Vector: pBluescript-EGFP
Insert size: 3215 bps
Insert sequence:CTGACCAGTT TTAGCCAATC AGACGATCTG GATCTTTGTT AACAAGCTAG GCATAAAGTT AAATATTAAA TAATATTGAA GACGTTTAGG TTGATTATAG TATTTTTGCC CCAGTGTTCT AAAATGAACA GTTTGTAAAA CATATTAAAT TTCTTTCTTT CATCGATTTT TTGCAGTATA TTGCTTATAT AATTTTGAAC CTATTTATAG TGCAATATGA AATTGTGTTT TTATTTAAAA CGTTTTCCAT ATTTCAATTT AATTTACAAA GGAAATACAA GATATCACAA AAGTAGGTGT TATTTTGGTT AGAACTCTAT ACAGCTTTAA ATAGGATTTC GCTTAAATAT CAACTATTAA CAATATTTAT AAAGGAATTT CTGTGGTTTA GTTAGGCGTT GATTGATTAG ATCTTAATTG CCATTTATAC CCTCAAGCAA TGTTGTGTTT GTGTATTGTT TCACATTTCT TTAGGTTGCA ATGTTACAAA CTCTTTAAAA AAAATTCATT TTTAAATTGG TTGCAGAAAC ATGGTTCGTG AAATCAACTG CTTGTAATAA ACAGGCTAAC AGGGTTTCTG CGTCATGTGA GAAAGAACTA TTTTGTAATT GTATCATAAA CCACAACGCA TATAATTAGG TTGGTAAAAC TTGTTTTGGG TTTGTTGTGG GAAGAATTCC TTTATTAAAT ATTACTGACT AAACACCGAT GACTCATATA CGTTTGAGAA AAACAGTGGC AAAGAGGTTA CATTTATCCC TTCGGTCAAA CGATGCAAAA ACATCGTAAA ATTTGAACTC ATTTTAACCA ATCAGAAACG ACCTCTCATT TTTCAGTGAC GTCATTTTTG CTGGTTGCTT TCTGGTACCC GCTATTTTTG AGATTATTCT TAACAATATT TTACTTTAAA AAAATACATA CATTGTAGAG AAAACTAGGT TAACACATTT ATATTAACTA AATTTGTTTT TGTTGCAAGT CCACCTATAC CCCAATTGCT TATCGCGGAT TGCGCGAAAA AAAAGAAAAA AAAAATCGAT TTTTGAAATG AGGTCATCAA GAACGAAAGA AGAAACGCGC AGAAGCGGAG TGGATCGATA GTTGGGCGTA TTCGGTTTGC GAGTGGCGTA CATTGCCGTT GTAATATTAG GTTTTCAGCT ATGTACGGTG TAATTGAGAG TGCTTAAAAT GTGAACACGT TAGTAAACAA TATTACTAAG TTCCGTGCTA AGAATAAGAA TCTAGGTCCT CCCTGTAGTG CGCTTATTTG TTGATTCTGT GAAATGATTT CCGATGTATG AGAACTGAGC GTTTGTATTT GTTTTCCCAT CATATAGTAG CCACTGCAAG TCCGTTGTAT TCATCAGTTG AAGTGTTATA AAGTGAGTGT AAAAGTGTTA TAAGGTAATT TTTAAAGTTA GAAGGCATTT CTGGGAAAAG TGTTAACTTA ATACAATATC TGGTTTTAAA GGTGTGCACA ATATACGGCC TTCAAATTGA CTATGGAAGT ATTTTACTAT GTAGTTTGCA AAGGTAAAAC TTTAATAGCA CTTAGATTTA GCCAGTTTGG TTTTGCTTTT GTATTAACTA CCTTATGTTT CGAGAGCTGG TATGCTCTCT TGTACACTTT TGTTCTATTA AAATAATTGT GGAGTATTTG TTGATTCAGG TAGTAATGGC TACAAAAACA ACAATTGAGA AAAGATTAAA AACAAAATGG TACCTTCAAC AGCAAATATA TATATATAGA ATGAATATTT ATTTTTTGCT TGCCTCAAAC CGAGTTGAAA ATATTGTGAT ATTTTTTGTC AAGATTTAAA AAGAATTTTT GAGCGGTCCC GATCTTTTCT TTAATTATTA AATTTAATAC AGCAGTATTG ATTACAAACG ACCTGTGCTT GGATTTTCTT CGGAATTAGA AAAAAATGCG CAGATTGTTT TGGAAAGCCG TAACTGGCGA TTAATTCCAA TCAGGGCGAT ATATACTTTT TTAGTGTTTG GATAATGGAT CAACTGTGAC CTAAATCTCT GCTCTTATGC CGTACATTTA GCCAGTCAGC CCATTTTTAA AAAACACGTC ACATTTAGTC TAAATTGCTT TGGTTTTGTC ATATGAAGTC TGGTTTGTCA TGCTATATAA TAATGTACTA TGATTTTGTG TATAATATAA AAACAGAGAA TTGCAATACT CAAGGTATGT TGGTTGCAAC ATCAGTCCTT AAAAGGATGA TTGCCTTTGT ATAGTCATAG CTTATAGATA TTGGGGTTAT TAAATAATTT GGGTTCGTAA AGCAATTTAT TATGTGACGT ATGTAAAATA AATTTAACGA ATATTGCCTT GTTGTTGATG TCAGTTTATG ACTCCTGATA AAGGCAATAG AACTGAATCC TGTCCTTTTA TTGTTGGATT TTATGGCATA TATAACCCAT GTGTTGGATG CAATAATTCA TTAACCCTCA TCATGTCAGT ATGCCACCAA AATGTTGATT ATTTGCTTCA AGGACCATTT CAAGATTTTT CTGTATAGAT TAATCAGGGC AACTAACAGC CTGTAAAATA ATTTGTTTTC CAAAATTAGT AATACTATAA TTATGTAAAG GACCTCTTAA TATATGAATA AGACTGCTTT GCGTAATGTA TATATTATAT CATGTTACCA TTTTAGAAAA GGTTCCTGTT AAAAGCAACT ATGGGTGATT TTTCACAATA TCAAATTATT AAATGACAAT TTGTTTCTAT ATCGAGTAAC TTGTGTGCGT ATATAAATAT CTAAGTAGTG TTTATATTCC AATCAACTTT GGCTAAACAA TTAGTGACGT ACACTGGTTA AGTATAGACC CTCTCAATTA GTATATACAG TAGGAGTGAT CTATTAAGAT AATTATTTAA TTACTTCAGT TGGATTCGTG TGCTTAACAA TGGATACTTG AGTACGTTTT GTTTTTGTAA TGCGTCAACT ACTCTGAGGT GACTCATTTA TAATATAAGT CTAATAGCCA GCCAGTTGTG TTACGTGTAT CTTGTATGTG ACTGCTTATA TACTTTAATC CTGTTATGTT CTCTGTTGTA GATTTGAGCT AAGCATTTTT ATTTTACGAT TTTATACAAA CTGGATATCT AAGAAAGAAG ACAAAGGTTG ATTTCCATTC TAACTTTTCT ATTTATCTAC AAAACCTTTC TACAGGACAC GGTGGTTTCA TCCCTTTGGT TTCAG