Version 2.0
D B T G R

DataBase of Tunicate Gene Regulation
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About DBTGR

Ci-G-alpha-i1

Gene product: G protein alpha (i) subunit
Organism: C. intestinalis
Summary of experimentsRegion from -4214 to -8 from the start codon allows expression in the adhesive organ, brain vesicle, visceral ganglion and dorsocaudal peidermal neurons.

From -4214 to -2873 is required for expression in the central nervous system and the dorsocaudal epidermal neurons.

From -2873 to -1564 is required for expression in the adhesive organ.

-3176 to -3090 is necessary for expression in the central nervous system and the dorso-caudal epidermal neurons, but not for expression in mesenchyme and adhesive organs.
Activity reported

An unknown factor regulates Ci-G-alpha-i1 in adhesive organ, brain vesicle, visceral ganglion, dorsocaudal epidermal neurons, neurons of the central nervous system and papilla, ocellus photoreceptor cells, cholinergic neurons, subpopulation of GABAergic neurons in C. intestinalis (1, 6, 21, 40)
Reporter construct expression
Name Gene Size Tissue Construct
Ci-G-alpha-i1 Ci-G-alpha-i1 4201 neurons in central nervous system, papillar neurons, mesenchyme
Ci-G-alpha-i1-basal promoter Ci-G-alpha-i1 470 not detected
Ci-G-alpha-i1-int Ci-G-alpha-i1 3215 papillal, brain vesicle, visceral ganglion, caudal epidermal neurons
Sequences available
ID Gene Description Organism Dataset Dataset ID
s00001 Ci-G-alpha-i1 Mapping of the Ci-G-alpha-i1 expression vector insert C. intestinalis JGI 1 blat
s00056 Ci-G-alpha-i1 Mapping of the Ci-G-alpha-i1-basal expression vector insert C. intestinalis JGI 1 blat
s00062 Ci-G-alpha-i1 Mapping of the Ci-G-alpha-i1-int expression vector insert C. intestinalis JGI 1 blat
s00082 Ci-G-alpha-i1 Mapping of the Ci-G-alpha-i1 expression vector insert C. intestinalis JGI 2 blat
s00133 Ci-G-alpha-i1 Mapping of the Ci-G-alpha-i1-basal expression vector insert C. intestinalis JGI 2 blat
s00139 Ci-G-alpha-i1 Mapping of the Ci-G-alpha-i1-int expression vector insert C. intestinalis JGI 2 blat
s00217 Ci-G-alpha-i1 -3000:+200 region of ci0100135237 C. intestinalis JGI 1 ci0100135237
s00218 Ci-G-alpha-i1 -3000:+200 region of ci0200209567 C. intestinalis JGI 2 ci0200209567
s00219 Ci-G-alpha-i1 -3000:+200 region of ENSCING00000005305 C. intestinalis JGI 2 ENSCING00000005305
References
  1. Kusakabe T., Yoshida, R., Ikeda, Y., Tsuda, M. (2004). "Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona." Dev Biol 276:563-580. (PUBMED)
  2. Kusakabe T. (2005). "Decoding cis-regulatory systems in Ascidians." Zoolog Sci 22:129-146. (PUBMED)
  3. Yoshida, R., Sakurai, D., Horie, T., Kawakami, I., Tsuda, M., Kusakabe, T. (2004). "Identification of neuron-specific promoters in Ciona intestinalis." Genesis 39:130-140. (PUBMED)
  4. Yoshida, R., Horie, T., Tsuda, M., Kusakabe, T. (2007). "Comparative genomics identifies a cis-regulatory module that activates transcription in specific subsets of neurons in Ciona intestinalis larvae." Dev Growth Differ 49:657-667. (PUBMED)