D B T G R
DataBase of Tunicate Gene Regulation
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About DBTGR
Ci-MLC2
Gene: Ci-MLC2
Ghost cluster: 3295;189
Reference:
Kusakabe T., Yoshida, R., Ikeda, Y., Tsuda, M. (2004). "Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona." Dev Biol 276 :563-580. (PUBMED )
This construct is available from Prof. Kusakabe.
Detected expression
Expression was detected in muscle .
Construct description
Name: pCi-MLC2-EGFP
5'-end primer with a PstI restriction site: GTCTGCAG GCCAAACACGTAAGGAATAC
3'-end primer with a BamHI restriction site: CTGGATCC TCTGCCTGTAGACAAAATCGA
Vector: pBluescript-EGFP
Insert size: 643 bps
Insert sequence:GCCAAACACG TAAGGAATAC CCCGATGGTA CATATTGGTA GCATTATGAA TGCTAATACA CAATGTTATT GTATGGTGAA AAGTGAAGAA CTTTTCACCA TACAATACAC TTTTTAGTGT AAAACTGTTT ATACAAATTT AATTTCGAAA TATTTAAAAA TCATTTTCCA TTCGCAAAGT GTGTAGTATC CAAGAACGTG ACTAAATAAA TATGCGCTCA AAACTTTACT CTTACTTTTC GAGGCTCATG CTGGTTGCGT TCAAGGACCA GAAGAAACAC ATAATCACCC GACCAAAGCG AAACAGCTTT GTTCACAACT AATCAAATTT CTTCCACCGC CTGTTCTCAC GTCACTAATT CCGCCTGTCT ATTCTGATGA CGTCATATAT CAGGTCATTA TGTCTGTTAT AAAAGCGCTG GGTCGAAGTT TAACAGCATT CAGTTCTGCT TAAGCATAAT CTAGCAAGCA TCAAACATCC TTCAAGACGA TAAACTATTA TAATGGTAAG TGTAGTTGTA TGCATTGCTG GGGAAGCACC AGTAGTAATA TACCATACCG ATAAAACTAG TTTAGATTGT TTAGAATTTT ATGTAGGATA AGCCTAAAAC TTAATAACAA CTGTCGATTT TGTCTACAGG CAG