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D B T G R

DataBase of Tunicate Gene Regulation
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About DBTGR

Ci-MLC2

Gene product: myosin essential light chain
Organism: C. intestinalis
Summary of experimentsRegion from -471 to +172 allows expression in muscle. (Kusakabe 2004)

TATA box is between -56 and -50.

The M2 motif at-110 is critical for expression in muscle and is the principal regulatory element for muscle expression.
The M2 motif at -79 is not important for expression in muscle. (Kusakabe 2004)

Ci-Tbx6b and Ci-Tbx6c bind to 5'-GWTCACACCT-3' in vitro.

Ci-Tbx6b is necessary for expression at the 110-cell stage and at the tailbud stage, Ci-Tbx6c is only necessary at the 110-cell stage (Yagi 2005)
Activity reported

Ci-Tbx6b regulates Ci-MLC2 in larval muscle in C. intestinalis (1, 2, 6)

Ci-Tbx6c regulates Ci-MLC2 in larval muscle in C. intestinalis (1, 2, 6)
Reporter construct expression
Name Gene Size Tissue Construct
Ci-MLC2-Basal Ci-MLC2 115 not detected
Ci-MLC2 Ci-MLC2 643 muscle
Sequences available
ID Gene Description Organism Dataset Dataset ID
s00059 Ci-MLC2 Mapping of the Ci-MLC2-Basal expression vector insert C. intestinalis JGI 1 blat
s00066 Ci-MLC2 Mapping of the Ci-MLC2 expression vector insert C. intestinalis JGI 1 blat
s00135 Ci-MLC2 Mapping of the Ci-fkh-Basal expression vector insert C. intestinalis JGI 2 blat
s00136 Ci-MLC2 Mapping of the Ci-MLC2-Basal expression vector insert C. intestinalis JGI 2 blat
s00280 Ci-MLC2 -3000:+200 region of ci0100146149 C. intestinalis JGI 1 ci0100146149
s00281 Ci-MLC2 -3000:+200 region of ci0200210065 C. intestinalis JGI 2 ci0200210065
References
  1. Kusakabe T., Yoshida, R., Ikeda, Y., Tsuda, M. (2004). "Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona." Dev Biol 276:563-580. (PUBMED)
  2. Yagi, K., Takatori, N., Satou, Y., Satoh, N. (2005). "Ci-Tbx6b and Ci-Tbx6c are key mediators of the maternal effect gene Ci-macho1 in muscle cell differentiation in Ciona intestinalis embryos." Dev Biol 282:535-549. (PUBMED)
  3. Kusakabe T. (2005). "Decoding cis-regulatory systems in Ascidians." Zoolog Sci 22:129-146. (PUBMED)