D B T G R
DataBase of Tunicate Gene Regulation
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About DBTGR
Ci-MRLC1
Gene: Ci-MRLC1
Ghost cluster: 31
Reference:
Kusakabe T., Yoshida, R., Ikeda, Y., Tsuda, M. (2004). "Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona." Dev Biol 276 :563-580. (PUBMED )
This construct is available from Prof. Kusakabe.
Detected expression
Expression was detected in muscle .
Construct description
Name: pCi-MRLC1-EGFP
5'-end primer with a PstI restriction site: CACTGCAG GCAGCAGCACAGCATATTGT
3'-end primer with a BamHI restriction site: GAGGATCC TTTCGTCCACCGGCCTAAGA
Vector: pBluescript-EGFP
Insert size: 570 bps
Insert sequence:GCAGCAGCAC AGCATATTGT AGCATTAATG TGATCAAAAT TGGGGTAATG GGCCACGTCT GGCCTAAATC CCAGGTCCTA ACGGACCTCA CCCATACAGA ATGTGATCAA AAAAGTTTCT TAATGATCGT TAGTTAACAA AATCCATTTA GCAAGGTGAT ATTGCTGTTC GTATAAGATT CTACATATTT TCAATTTCGT CATCGCAAAA GCAGTGTTGG ATCGACGCGG GTTGCCTATA GTACGATTTA TAACACTATT TATCTTGACA CGTTGCGCTG AACAATGTAG AGACTGATAT ATGTTGCTTG AGTTTGCGCA ACGTGACAAG CGCATCGCCC CTGCATATCA AGAATGTGAC GACAGCAGTG TTGTCACTTA CATCGTTTGT GGGGTATAAA AGGCGAGGCC CGCCTGGTAA GAAATCATTC CAACTATTTG ACTCTTCAGC TTACTTGCTT CTTCAGCTAA CCAGACAAGG AATTCTTAAA ATGGTAAGTT GTAACATTCT CATACCGATC AAGAGATAGA GTTTAAAACT GACTTTGAAT TCTTAGGCCG GTGGACGAAA