Version 2.0
D B T G R

DataBase of Tunicate Gene Regulation
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About DBTGR

Ci-MRLC1

Gene product: myosin regulatory light chain
Organism: C. intestinalis
Summary of experimentsNote: This gene is named Ci-MRLC2 in the publications by Kusakabe et al.

Region from -434 to +136 allows expression in muscle. (Kusakabe 2004)

TATA box between -39 and -34.

The M2 motif is important for expression in muscle. (Kusakabe 2004)

Ci-Tbx6b and Ci-Tbx6c bind to 5'-GWTCACACCT-3' in vitro.

Ci-Tbx6b and Ci-Tbx6c are necessary for expression at the 110-cell stage. (Yagi 2005)
At the tailbud stage, in the absence of Ci-Tbx6b, expression is only found in the posterior blastomeres (Yagi 2005)
Activity reported

Ci-Tbx6b regulates Ci-MRLC1 in larval muscle in C. intestinalis (1, 2, 6)

Ci-Tbx6c regulates Ci-MRLC1 in larval muscle in C. intestinalis (1, 2, 6)
Reporter construct expression
Name Gene Size Tissue Construct
Ci-MRLC1 Ci-MRLC1 570 muscle
Sequences available
ID Gene Description Organism Dataset Dataset ID
s00067 Ci-MRLC1 Mapping of the Ci-MRLC1 expression vector insert C. intestinalis JGI 1 blat
s00143 Ci-MRLC1 Mapping of the Ci-MRLC1 expression vector insert C. intestinalis JGI 2 blat
References
  1. Kusakabe T., Yoshida, R., Ikeda, Y., Tsuda, M. (2004). "Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona." Dev Biol 276:563-580. (PUBMED)
  2. Yagi, K., Takatori, N., Satou, Y., Satoh, N. (2005). "Ci-Tbx6b and Ci-Tbx6c are key mediators of the maternal effect gene Ci-macho1 in muscle cell differentiation in Ciona intestinalis embryos." Dev Biol 282:535-549. (PUBMED)
  3. Kusakabe T. (2005). "Decoding cis-regulatory systems in Ascidians." Zoolog Sci 22:129-146. (PUBMED)