Copyright © 2005-2008, Human Genome Center,
Inst. Med. Sci., Univ. Tokyo. All rights reserved.
Version 2.0
D B T G R
DataBase of Tunicate Gene Regulation
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eg: snail, muscle, ci0100133445, ...
names
tissues
transcription factors
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eg: chr09q:3065382-3068581, ...
C. intestinalis
1.0
C. intestinalis
2.0
C. savignyi
1.0
C. savignyi
2.0
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About DBTGR
Ci-TnT
Gene product: troponin T
Organism:
C. intestinalis
Summary of experiments
At most 600 bp are necessary (Davidson 2003)
Ci-Tbx6b binds to 5'-GWTCACACCT-3' in vitro.
Ci-Tbx6b is necessary for expression at the 110-cell stage. (Yagi 2005)
At the tailbud stage, in the absence of Ci-Tbx6b, expression is only found in the posterior blastomeres (Yagi 2005)
Activity reported
Ci-Tbx6b
regulates Ci-TnT in
larval tail muscle
,
trunk ventral cells
,
central nervous system
,
brain vesicle
in
C. intestinalis
(
1
,
2
,
5
,
6
)
Reporter construct expression
Name
Gene
Size
Tissue
Construct
Ci-TnT
Ci-TnT
441
muscle
Sequences available
ID
Gene
Description
Organism
Dataset
Dataset ID
s00069
Ci-TnT
Mapping of the Ci-TnT expression vector insert
C. intestinalis
JGI 1
blat
s00145
Ci-TnT
Mapping of the Ci-TnT expression vector insert
C. intestinalis
JGI 2
blat
s00376
Ci-TnT
-3000:+200 region of ci0100146672
C. intestinalis
JGI 1
ci0100146672
References
Kusakabe T., Yoshida, R., Ikeda, Y., Tsuda, M. (2004). "Computational discovery of DNA motifs associated with cell type-specific gene expression in Ciona."
Dev Biol
276
:563-580. (
PUBMED
)
Yagi, K., Takatori, N., Satou, Y., Satoh, N. (2005). "
Ci-Tbx6b
and
Ci-Tbx6c
are key mediators of the maternal effect gene
Ci-macho1
in muscle cell differentiation in
Ciona intestinalis
embryos."
Dev Biol
282
:535-549. (
PUBMED
)
Davidson, B., Levine, M. (2003). "Evolutionary origins of the vertebrate heart: specification of the cardiac lineage in Ciona intestinalis."
Proc Natl Acad Sci USA
100
:11469-11473. (
PUBMED
)
Kusakabe T. (2005). "Decoding
cis
-regulatory systems in Ascidians."
Zoolog Sci
22
:129-146. (
PUBMED
)